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PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication
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Plant-DirectTM PCR Kit contains all the reagents needed for quick preparation of plant genomic DNA and PCR amplification with the prepared DNA samples. Any plant tissues can be used such as leaf, stem, root or cultured plant tissues. This kit can be applied to any plant species as long as correct primers are used. The PCR products can be directly loaded onto agarose gel without addition of loading dye for visualization of the experimental results.
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Advantage 2 Polymerase Mix is a hot-start PCR polymerase mix that contains Takara's Titanium Taq DNA Polymerase (with TaqStart Antibody), and a minor amount of a proofreading polymerase for a balance of higher yield and higher fidelity than wild-type Taq. The Mix is ideal for amplification of long templates up to 18 kb and complex genomic DNA up to 6 kb. Two different optimized buffers are supplied with the polymerase mix. dNTPs need to be purchased separately. Enough enzyme mix and PCR buffer are supplied for 100 PCR reactions of 50 μl each.
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ReadyMix(TM) Taq PCR Reaction Mix is a prepared SOLUTIONon containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigmas high quality Taq DNA Polymerase 99% pure deoxynucleotides and buffer in a 2x optimized reaction concentrate. for reaction set-up add the ReadyMix (25 muL) to the primers template and water (total volume 50 muL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigmas Taq DNA Polymerase.
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